Permit inoculum to generally be fully absorbed just before incubating for 24 h at thirty°C. Test plates for lecithinase generation as indicated by zone of precipitation encompassing growth. Mannitol isn't fermented by isolate if progress and surrounding medium are eosin pink. (Yellow colour signifies that acid is created from mannitol.) B. cereus colonies tend to be lecithinase-beneficial and mannitol-adverse on MYP agar.
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